Fig. 1.

Mouse livers contain both high (H)-lipid and low (L)-lipid subpopulations of hepatocytes. A: C56BL/6 male mice (11–12 wk) were fed a normal chow diet. Triacylglycerol (TAG) levels were measured in hepatocytes freshly isolated from mice under fed conditions and normalized to total protein levels (n = 4). B–D: primary hepatocytes were cultured for 10 h. B: TAG levels (n = 4). C: representative image of Oil red O staining of hepatocytes from 4 similar experiments. D: hepatocyte extracts were immunoblotted with antibodies against albumin or tubulin. Values are presented as means ± SE. *P < 0.05.