Fig. 4.

H-lipid hepatocytes are more susceptible to injury and death than L-lipid hepatocytes. Primary L-lipid and H-lipid hepatocytes were treated with or without 0.1 μg/ml LPS and 0.25 mM PA for 24 h. A: alanine transaminase (ALT) activity in growth medium was measured and normalized to total hepatocyte protein levels (n = 5). B: hepatocyte viability was measured using MTT assays (n = 4). C: hepatocyte apoptosis was examined by TUNEL assays. A representative image is shown. D: TUNEL-positive cells were quantified and normalized to total cell numbers (n = 4). E: hepatocyte extracts were immunoblotted with antibodies against caspase-3 (casp-3) or tubulin. Levels of active caspase-3 were quantified by densitometry and normalized to tubulin levels (n = 5). Values are presented as means ± SE. *P < 0.05.