Fig. 6.

HFD consumption selectively increases expression of proinflammatory cytokines in the H-lipid subpopulation. C57BL/6 male mice (9–10 wk) were fed Chow or HFD for 2 wk. A: primary L-lipid and H-lipid hepatocytes were treated with 0.1 μg/ml LPS and 0.25 mM PA for 4 h. Expressions of indicated genes were measured by qPCR and normalized to 18S expression (n = 5). B: conditioned media were prepared from LPS/PA-treated L-lipid and H-lipid hepatocytes. BMDMs were stimulated with indicated conditioned medium for 5 h, and expressions of the indicated genes were measured by qPCR and normalized to 18S expression (n = 5). Values are presented as means ± SE. *P < 0.05.