Fig. 1.
Angiotensin type 2 receptor (AT2R) and angiotensin type 1 receptor (AT1R) protein and mRNA expressions. A: relative levels of AT2R and AT1R proteins in various tissues of adult male rats. Brain stem was employed as a standard to normalize the expression level. A1, glandular tissues; A2, muscular tissues; A3, general tissues. *P < 0.05 and **P < 0.01 compared with brain stem; n = 6 animals/group. B: relative expression levels of AT2R and AT1R mRNA in various tissues compared with the brain stem in adult male rats. Tissue Codes: 1, brain stem; 2, thyroid; 3, thymus; 4, pancreas; 5, adrenal; 6, testicle; 7, parathyroid; 8, epididymis; 9, seminal vesicle; 10, heart; 11, diaphragm; 12, skeletal muscle; 13, intestine; 14, bladder; 15, aorta; 16, brain microvessel; 17, lung; 18, spleen; 19, liver; 20, kidney; 21, skin; 22, eye; and 23, fat. *P < 0.05 and **P < 0.01 compared with brain stem; n = 4/group. C: AT2R protein in pancreas. B1, Western blot showing AT2R expression in the extract of whole pancreas, islet, and acinar. B2, immunofluorescence staining showing localization of AT2R protein in islets and acinar cells. D: ontogeny of AT2R and AT1R proteins in brain stem (D1), testicle (D2), and pancreas (D3). **P < 0.01 compared with day 1 or day 3; n = 5/group. d, day; w, wk after birth.