Table 1.
Gene-specific primers and probes for real-time PCR (from Integrated DNA Technologies)
| Name of Genes | Forward Primers | Reverse Primers | Probes |
|---|---|---|---|
| AT2R | CCCTTCCTGTATTGTTTCGTTG | GACATAGTCTCTCTCTTGCCTTG | 56-FAM/AGCTCCGTA/ZEN/GTGTGTTTAGAGTTCCCA/3IABkFQ |
| AT1aR | TCCTGTTCCACCCGATCA | GCCAGCCATTTTATACCAATCTC | 56-FAM/CAGCTCTGC/ZEN/CACATTCCCTGAGT/3IABkFQ |
| Proinsulin 2 | CAAGCAGGTCATTGTTCCAAC | GTAGAGAGCTTCCACCAAGTG | 56-FAM/CCAGAGGAT/ZEN/GAGCAGGGCCAG/3IABkFQ |
| GAPDH | AACCCATCACCATCTTCCAG | CCAGTAGACTCCACGACATAC | 56-FAM/CAGCACCAG/ZEN/CATCACCCCATTTG/3IABkFQ |
AT2R, angiotensin type 2 receptor; AT1aR, angiotensin type 1a receptor.