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Human islets do not form CaP layers when cultured in DMEM. SEM of human islets after silica coating followed by 7 days of culture in different media conditions. Human encapsulated islets incubated in DMEM (A) do not form a secondary CaP layer, whereas those incubated in RPMI (B) develop CaP deposits. White arrow indicates portion of exposed islet surrounded by the CaP encapsulant.
