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Proinflammatory mRNA expression in reconventionalized WT and NHE3 KO. Real-time PCR analysis of TNF, IL-1β, iNOS, MMP8, and interferon (IFN)-γ mRNA expression in the distal colon of reconventionalized WT and NHE3 KO mice. Data were normalized to GAPDH mRNA as an internal control and calculated using ΔΔCt method. Statistical analysis was performed with ANOVA followed by Fisher's PLSD post hoc test with P ≤ 0.05 considered significant (reconventionalized WT n = 5 and NHE3−/− n = 7).
