Table 1.

Primer pairs for PCR amplification of TRPV1 and P2X3 mRNA

Gene (expected size)	External Primers	Internal Primers	Genebank Number
TRPV1	GGGAAGAATAACTCACTGCCTGTG	GGCGAGACTGTCAACAAGATTGC	NM_001001445.1
(486, 191 bp)	TGGGTCCTCGTTGATGATGC	TCATCCACCCTGAAGCACCAC
P2X3	GCTCCGTAGAAGAAGATGGAGA	TGTCCTAAGAGGATCCTGTACC	NM_145526
(251, 141 bp)	CTGTGTGACCATGTTAGGGATG	GGCATCTAGCACATAGAAGTGG

Single-cell PCR was performed in dissociated colorectal afferent somata from L6-S2 dorsal root ganglions (LS DRGs). A nested PCR strategy with external and internal cDNA primer pairs was used to amplify reverse-transcribed transient receptor potential vanilloid 1 (TRPV1) and P2X3 mRNA. Sequences for cDNA primers are indicated with the 5′ end on the left. The Genebank reference used for designing primers is indicated at the right. bp, base pairs.