Table 2.
Expression of TRPV1 and P2X3 mRNA and response to channel agonists in lumbosacral colon sensory neurons
| mRNA Expression |
Response to Channel Agonists |
|||
|---|---|---|---|---|
| % | n | % | n | |
| TRPV1 only | 77% | (10/13) | 74% | (23/31) |
| P2X3 in TRPV1 positive neurons | 80% | (8/10) | 30% | (7/23) |
| P2X3 only | 85% | (11/13) | 35% | (11/31) |
| TRPV1 in P2X3 positive neurons | 67% | (8/11) | 64% | (7/11) |
A total of 14 LS DRG sensory neurons innervating the colorectum (c-DRG) including 1 control from 1 preparation were tested for mRNA expression. A total of 31 LS c-DRGs from 2 preparations was examined for Ca2+-transient responses to α,β-meATP (100 μM) followed by capsaicin (500 nM). For Ca2+ imaging, only cells that initially responded to 30 mM KCl were treated with agonists.