Fig. 5.

Western blots showing inhibition of PDGF-α receptor (A) and PDGF-β receptor (B) phosphorylation by imatinib. Even-numbered lanes received imatinib (IB). Lanes 1 and 2, control; lanes 3 and 4, PDGF-CC; lanes 5 and 6, PDGF-AA, lanes 7 and 8, PDGF-BB; lanes 9 and 10, PDGF-AB. Black lines separate different Western blot membranes using samples from the same culture of vascular smooth muscle cells. C: comparison of effect of tyrosine kinase inhibition with imatinib on increases in thymidine incorporation (DNA synthesis) in rat PASMCs exposed to PDGF-BB, -AB, and -AA. n = 3–5 experiments. *P < 0.05.