Fig. 6.

Expression of periostin and FSP1 mRNA by CD11b⁺ myeloid cells and by CD11b-negative (nonmyeloid cells) isolated from control and infarcted wild-type mouse hearts. A: CD11b⁺ and CD11b-negative cells isolated from control hearts show low levels of periostin mRNA expression. CD11b-negative cells harvested after 24 h and 7 days of reperfusion exhibit markedly increased periostin mRNA synthesis (^∧∧P < 0.01, ^∧P < 0.05 vs. control). CD11b⁺ myeloid cells have no increase in periostin mRNA levels after 24 h, but exhibit significantly increased expression after 7 days of reperfusion (**P < 0.01 vs. control CD11b⁺ cells). Periostin expression levels in cardiac CD11b-negative cells are significantly higher than in myeloid cells at the same time point (*P < 0.05 vs. corresponding CD11b⁺ cells). B: FSP1 expression is very low in nonmyeloid cells harvested from control hearts, but is increased significantly in nonmyeloid cells isolated after 24 h of reperfusion (**P < 0.01 vs. control). CD11b⁺ myeloid cells from control hearts exhibit high FSP1 mRNA expression, at levels significantly higher than corresponding nonmyeloid cells (^∧P < 0.05). Myeloid cells isolated from infarcts after 7 days of reperfusion also have significantly higher levels of FSP1 mRNA expression than corresponding nonmyeloid cells.