Fig. 1.

Melting point profiles for α₃ (A), α₇ (B), and β₄ (C) neuronal nicotinic ACh receptor (nAChR) subunits as well as postsynaptic density (PSD)-93 (D). The amplified product for each gene was subjected to SYBR green I melting analysis by ramping the temperature of the reaction samples from 60 to 95°C. A single DNA melting profile was observed under these dissociation assay conditions, demonstrating amplification of a single unique product, free of primer dimers or other anomalous products. Melting temperatures of the quantitative real-time PCR products were 82.6, 85.1, 85, and 79.7°C for α₃, α₇, β₄, and PSD-93, respectively. NTC equals no template control.