Fig. 3.

nAChR subunit and PSD-93 transcript levels are decreased 3 days after cavernous nerve axotomy (A), crush (B), or manipulation (C). All transcript levels were normalized to the transcript level for L32, and the normalized transcript levels from the ipsilateral (Ipsi) operated side are expressed as a fraction of normalized transcript levels in the contralateral unoperated MPG, which served as a control (Ctl). A and B: after cavernous nerve axotomy or crush, transcript levels for α₃, β₄, and PSD-93 were significantly decreased (P = 0.002 in A,1, P = 0.01 in B,1, P = 0.04 in A,2, P = 0.018 in B,2, P = 0.01 in A,4, and P = 0.05 in B,4), whereas α₇ transcript levels were not changed (P = 0.89 in A,3 and P = 0.18 in B,3). C: after dissection/stretch (manipulation), transcript levels for α₃ and PSD-93 were significantly decreased (P = 0.003 in C,1 and P = 0.02 in C,4), whereas β₄ and α₇ transcript levels were not significantly changed (P = 0.26 in C,2 and P = 0.69 in C,3). Data are means ± SE of transcript levels determined in MPG extracts from 4–6 animals/group.