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Pressure-induced increases in mitochondrial ROS production in isolated, cannulated branches of the mouse middle cerebral artery. Data are relative changes in the buildup of MitoSox fluorescence intensities induced by stepwise increase in intraluminal pressure. Note that high pressure upregulates vascular mitochondrial ROS generation. MitoSox loading was performed as previously reported (26). Data are means ± SE (n = 6 in each group). *P < 0.01.
