Fig. 8.

Effect of CCR2 on neutrophil traffic in mice with experimental Gram-negative pneumonia. A–C: comparison of neutrophil numbers in the bone marrow, blood, and lung compartments at various times after intrapulmonary challenge with Klebsiella. Day 0 represents uninfected mice. NS, no significant difference between wild-type and CCR2-deficient hosts (2-way ANOVA). D and E: representative lung histological sections from wild-type and CCR2-deficient mice on day 3 of infection, stained with hematoxylin and eosin (original magnification ×40, scale bar = 100 μm) show alveolar consolidation. Insets: (original magnification ×400, scale bar = 50 μm) most of the intra-alveolar cells are neutrophils. F: lung myeloperoxidase activity on day 3 of infection. Each data point represents 1 animal and horizontal lines indicate medians; *P < 0.05 compared with wild-type group (Mann-Whitney).