Fig. 6.

Bortezomib induces autophagy in human glioblastoma U87 and U251 cells. a Western blot analysis for the expressions of LC3 and Beclin 1 in U87 cells treated by 0, 5, 10, and 15 nM bortezomib for 24 h and the expression of LC3 in U87 cells treated by bortezomib (10 nM) alone or together with Bafilomycin A1 (1 μM) for 24 h. b Quantitation of LC3-II and Beclin 1 proteins levels in U87 cells treated by 0, 5, 10, and 15 nM bortezomib for 24 h. c Western blot analysis for the expression of LC3 and Beclin 1 in U251 cells treated by 0, 5, 10, and 15 nM bortezomib for 24 h and the expression of LC3 in U251 cells treated by bortezomib (10 nM) alone or together with Bafilomycin A1 (1 μM) for 24 h. d Quantitation of LC3-II and Beclin 1 proteins levels in U251 cells treated by 0, 5, 10, and 15 nM bortezomib for 24 h