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. 2013 Nov 26;7:232. doi: 10.3389/fncel.2013.00232

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Copyright © 2013 Shrivastava, Rodriguez, Triller and Renner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of transfected P2X7-Dendra2 receptors in dendrites. Dendra2-tagged P2X7Rs were transfected in hippocampal neurons at DIV 12. Forty-eight hours after transfection, immunocytochemistry was performed to determine the localization of P2X7Rs. (Aa,Ba,Ca) Diffused labeling of P2X7Rs with expression in cell body and neurites can be observed. (Aa–Ac) Labeling of axons with tau antibody shows axons running apposed to the processes expressing P2X7-Dendra2 receptors. (Ba–Bc) MAP2 labeling of dendrites show an overlap with P2X7-Dendra2 expressing neurites. (Ca–Cc) Synapse labeling with pre-synaptic synapsin protein shows pre-synaptic boutons are apposed to P2X7-Dendra2 expressing processes.

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