Figure 2.

PALM reveals nanoclusters of P2X7Rs. PALM imaging was performed on live (A) and fixed (B) neurons transfected with P2X7-Dendra2. (Aa,Ba) Representative fluorescent images of a section of dendrite before PALM imaging was performed. (Ab,Bb) Pointillist images displaying all detections obtained for P2X7-Dendra2. (Ac,Bc) Super-resolved rendered image generated with a pixel size of 5 nm. Note fixation-induced clustering in (Ab,Ac). (Bd) Boxed-region 1 and 2 in (Bc) at higher magnification shows predominantly random, but also some nanoclusters of P2X7-Dendra2 receptors. (Be) Two random nanoclusters, “cluster a” from box 1 and “cluster b” from box 2, shown at higher magnification. Each detection within clusters “a” and “b” is represented as a blue vertical line and plotted as a function of time (x-axis).