Figure 5.

ATP increased the mobility of P2X7Rs. Live cell sptPALM was performed either in the absence or presence of ATP (100 μM) in recording medium. (A–C, Table 1) ATP differentially increased the diffusion coefficient of “free” WT and K17A-mutant P2X7Rs but not of K64A-mutant receptors. (D–F, Table 1) No change in the diffusion coefficient of “trapped” receptors was observed for WT and mutant-P2X7Rs. Inset (A–F) shows no change in MSD under any condition. (Kolmogorov-Smirnov test. ^***p < 0.001, ^*p < 0.05). Median diffusion coefficient and number of trajectories are shown in Table 1.