Table 1.

(Supporting Figure 5) Median diffusion coefficient (μm²/s) and number of trajectories (>10 points) used for diffusion calculation.

		Control median D (μm2/s	+ATP median D (μm2/s)
WT-P2X7Rs	Non-nanoclusters	0.0910 (n = 597)	0.1087 (n = 403)*
	Nanoclusters	0.0381 (n = 327)	0.0367 (n = 365)ns
K17A-P2X7Rs	Non-nanoclusters	0.0422 (n = 270)	0.0855 (n = 143)***
	Nanoclusters	0.0230 (n = 109)	0.0192 (n = 92)ns
K64A-P2X7Rs	Non-nanoclusters	0.1435 (n = 472)	0.1247 (n = 504)ns
	Nanoclusters	0.0661 (n = 420)	0.0570 (n = 327)ns

Kolmogorov-Smirnov test between Control and ATP treated cells.