. Author manuscript; available in PMC: 2013 Nov 26.

Published in final edited form as: Arch Biochem Biophys. 2008 Mar 14;474(1):10.1016/j.abb.2008.03.008. doi: 10.1016/j.abb.2008.03.008

Table 2.

Role of ATP hydrolysis in hepatic mitochondrial GSH uptake

Addition	GSH uptake (nmol/min per mg protein)
Addition	− AA	+ AA
Buffer	1.64 ±0.14	2.05 ±0.31
+2 mM ATP	2.79 ± 0.67^*	2.27 ± 0.41
+ATP + Oligomycin (20 µg/ml)	2.55 ±0.13^*	2.50 ±0.13
+ATP + Atractyloside (20 µM)	1.93 ±0.23	2.61 ±0.15^*

Initial rates of uptake of 7.5 mM GSH in suspensions of isolated mitochondria from rat liver were measured with L-[³H-glycyl]-GSH with the indicated additions and in the absence and presence of 2 µM antimycinA (AA). Measurements are means ± SEM of 5 experiments.

P < 0.05 compared with corresponding control value.