Table 3. Comparison of most commonly used RAD sequencing methodologies and associated costs.
| No. of enzymes |
Cut frequency |
Shearing required |
Size selection |
Library prep time & required expertise |
Initial outlay cost |
Subsequent library cost per sample |
Scalability to reduce overall cost per sample |
|
|---|---|---|---|---|---|---|---|---|
| ezRAD | 1 or more | Frequent | No | Yes | Low | Very Low | Moderate | Low |
| RAD tags | 1 | Rare | Yes | Yes | High | High | Low | Low |
| GBS | 1 | Rare or frequent |
No | No | Moderate | High | Moderate to very low |
Low |
| 2-enzyme GBS |
2 | Rare + frequent |
No | No | Moderate | High | Moderate to very low |
Low |
| ddRAD | 2 | Frequent | No | Yes | Moderate | High | Very low | Moderate |
| 2b-RAD | 1 | Frequent | No | No | Moderate | High | Low | Moderate |