FIG. 4.

Effect of specific inhibitors on T cell proliferation. Human purified T cells were stained with CFSE, stimulated with mitogenic anti-CD3 and anti-CD28 antibodies, and cultured alone or in presence of different resting and primed SC types (at 10:1 T/SCs ratio). In each coculture the following inhibitors (I) were added: L-1MT (A), Anti-IFN-γ (B), NS-398 (C), and snPP (D). After 6 days, cells were harvested and T cell proliferation was evaluated by FACS analysis. The results are expressed as relative proliferation percentage of T cells, normalized to T cells cultured alone (100%). Error bars represented mean±SD of five independent experiments for BM-MSCs, OE-MSCs, LeSCs, and AFSCs and three independent experiments for CSCs and LSCs. *P<0.05, **P<0.01, ***P<0.001.