Figure 6. Treg-mediated suppression of anti-hFIX formation is vector dose dependent.

1. Vector injected (2 × 10⁹, 1 × 10¹⁰ or 1 × 10¹¹ vg AAV8-ApoE/hAAT hF9) C3H/HeJ and naïve mice were Treg donors. Following purification using magnetic activated cell sorting 1 × 10⁶ Treg were injected into naïve recipient C3H/HeJ mice which were challenged the following by with 1 IU hFIX protein in adjuvant.
2. Anti-hFIX IgG1 (ng/ml) titres 3 weeks following subcutaneous challenge of hFIX in adjuvant. Data (average ± SD) is from vector treated Treg (n = 4 per vector dose) and naïve Treg controls (n = 8). The Student's 2-tailed T test was used to determine statistical significance (naïve vs. 10¹⁰ p = 0.0022, naïve vs. 10¹¹ p = 0.0008 and 10¹⁰ vs. 10¹¹ p = 0.0286).