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. 2013 Sep 24;5(11):1710–1719. doi: 10.1002/emmm.201302935

Figure 1. Gene delivery to mouse cortical and spinal neurons using AAV9 vectors.

Figure 1

  1. Large motor neurons (anterior horn cells, AHCs) expressed GFP in the spinal cords of wild-type mice injected in the tail vein with AAV9-GFP (1.5 × 1011 vg/body, n = 4). Scale bars, 50 µm (upper panels) and 20 μm (lower panels).
  2. Expression of Flag protein was observed in the AHCs of AR2 mice injected with AAV9-Flag-hADAR2 in the tail vein (AAV; 2.1 × 1012 vg/body) but not in mice injected with saline (Saline). Arrows indicate Flag-positive AHCs. Scale bars, 50 and 20 μm (insets).
  3. RT-PCR demonstrated the expression of Flag-hADAR2 in the anterior horn of AAV9-treated AR2 mice.
  4. Representative immunofluorescence images of the spinal cords of AR2 mice treated with AAV9-Flag-hADAR2 showing the expression of Flag (green) and choline acetyltransferase (ChAT) (red) protein using anti-Flag and anti-ChAT antibodies. Magnified view of the boxed areas in Supporting Information Fig S3A. Scale bar, 20 μm.
  5. Flag-hADAR2 did not colocalize with GFAP. TO-PRO-3 was used as a cell marker. Scale bar, 20 μm.
  6. Flag-hADAR2 did not colocalize with Mac2. TO-PRO-3 was used as a cell marker. Scale bar, 20 μm.