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. 2013 Sep 24;5(11):1710–1719. doi: 10.1002/emmm.201302935

Figure 4. ADAR2 gene expression in the anterior horn of the spinal cord (AH).

Figure 4

  1. The relative abundance of mouse ADAR2 mRNA did not significantly differ between AR2 mice injected with AAV9-Flag-hADAR2 (AAV) and those injected with saline (Saline; n = 5 for each group). hADAR2 mRNA was expressed at a significant level in AAV. The relative abundance of total (human and mouse) ADAR2 mRNA was 1.5-fold higher in AAV (n = 4) than in Saline (n = 4). **p < 0.01 (Mann–Whitney U-test). All error bars represent the s.e.m.
  2. The relative abundance of hADAR2 mRNA standardized with mouse ChAT mRNA indicated that there was significant expression of hADAR2 in ChAT-positive AHCs in AAV. n = 15 (AAV) and n = 6 (Saline). All error bars represent the s.e.m.
  3. The editing efficiency at the GluA2 Q/R site in the AH lysates (four samples from each mouse) from the AAV group (n = 8) was significantly higher than that in the control group (n = 7) (95.20 ± 0.36% vs. 90.68% ± 0.55%; Mann–Whitney U-test, ***p < 0.0001; (p = 0.0128 when compared between AAV and saline groups)). All error bars represent the s.e.m.