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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Methods. 2012 Dec 24;59(3):10.1016/j.ymeth.2012.12.007. doi: 10.1016/j.ymeth.2012.12.007

Table 1.

Thermodynamic and kinetic parameters from the distribution analyses of the different surfaces

surface immob (RU) total signal (RU) rmsd (RU) major peak (RU) Kd (nM) koff (10-3 s-1) kon (106 M-1s-1) minor peak (RU) peak ratio minor/major activity* (%) log10(ktr)
C1 800 15 n.d. 12
CM3 1350 86 0.30 37 1.2 1. 9 1. 6 42 1.13
CM3 3200 110 0.36 62 1.0 1.6 1.6 32 0.52 22
CM3 4100 100 0.40 82 1.1 1.4 1.3 8 0.10 16
CM5 3000 176 0.73 74 3.2 2.3 0.72 90 1.21 38
CM5 6000 461 1.59 254 2.4 1.2 0.49 183 0.72 50 9. 7
C1-SA 1500 175 0.83 78 0.80 1.1 1.4 73 0.94 75
CM3-SA 800 68 0.32 44 0.90 1.4 1.5 14 0.32 55
CM3-SA 2600 221 0.71 120 0.96 1.1 1.1 41 0.34 55 13 .1
CM5-SA 1000 143 0.71 143 1.4 1.7 1.3 43 0.30 93
CM5-SA 2000 135 0.76 97 2.2 1.9 0.87 - - 44 7. 8
*

The activity is the ratio of maximum calculated total binding signal at saturation to the predicted binding capacity based on the immobilization signal, the molecular mass of the analyte and the ligand, and the binding stoichiomtry.