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. Author manuscript; available in PMC: 2013 Nov 26.
Published in final edited form as: Science. 2010 May 13;328(5985):10.1126/science.1189123. doi: 10.1126/science.1189123

Figure 2.

Figure 2

MiR-33 regulates the cholesterol transporter ABCA1. A. Transfection of siRNA against Drosha and Dicer results in increased protein levels of ABCA1 in human HepG2 hepatoma cells (top row), human IMR-90 fibroblasts (middle row), and the mouse macrophage cell line J774 (bottom row). B. Introduction of miR-33a or miR-33b precursors into the three cell lines represses ABCA1 protein levels. C. MiR-33a/b antisense oligonucleotides (A33a/b) increase ABCA1 levels. E. Insertion of the ABCA1 3’UTR fragment into a Luciferase reporter results in decreased Luciferase expression in HEK293 cells. F. Co-transfection with wild-type, but not mutated, miR-33a/b precursors causes further repression of the Luciferase-ABCA1 3’UTR reporter. A scrambled sequence was used as precursor control (PC). G. MiR-33a/b antisense oligonucleotides result in de-repression of the Luciferase-ABCA1 3’UTR reporter. A scrambled sequence was used as antisense control (AC). Error bars represent S.D. * denotes p<0.05.