Table 3.
Method validation for the quantitative determination of six phenolic compounds using RP-HPLC
| Peak no. | Compounds | Regression equation |
Precision experiment |
Repeatability |
Recovery experiment |
|||
|---|---|---|---|---|---|---|---|---|
| Area of peak | RSD (%) | Area of peak | RSD (%) | Average recovery rate (%) | RSD (%) | |||
| 4 |
(+)-catechin |
y = 2004.7x - 25.13(R2 = 0.9973) |
83.12 ± 0.61 |
0.73 |
12.82 ± 0.22 |
1.74 |
101.54 ± 3.08 |
1.03 |
| 7 |
Caffeic acid |
y = 146577x + 12.70(R2 = 0.9998) |
323.05 ± 1.83 |
1.32 |
285.04 ± 2.94 |
2.94 |
102.66 ± 2.96 |
2.88 |
| 10 |
Ferulic acid |
y = 141714x - 14.70(R2 = 0.9998) |
416.81 ± 0.92 |
1.88 |
32.85 ± 0.87 |
1.32 |
105.95 ± 2.44 |
2.3 |
| 13 |
Hyperoside |
y = 66172x + 37.96(R2 = 0.9986) |
960.32 ± 5.22 |
2.11 |
5801.02 ± 17.51 |
2.66 |
105.72 ± 1.56 |
1.48 |
| 14 |
Rutin |
y = 43663x + 30.06(R2 = 0.9964) |
89.79 ± 0.65 |
0.76 |
239.68 ± 7.85 |
0.65 |
96.21 ± 0.34 |
0.36 |
| 16 | Ellagic acid | y = 62654x - 82.44(R2 = 0.9999) | 101.25 ± 1.43 | 0.93 | 181.42 ± 2.40 | 3.28 | 102.60 ± 1.20 | 1.17 |
Values were expressed in mean ± SD (N = 6).