Table 2.
COX-1, COX-2, and EGFR mRNAs and corresponding protein content in human colon mucosa compared to tumor tissue
| |
Tumor tissue |
ANOVA |
|||||
|---|---|---|---|---|---|---|---|
| Mucosa | Stadium I | Stadium II | Stadium III | Stadium IV |
Tumor vs mucosa p value |
Stage I-IV p value |
|
|
RNA (n) |
(26) |
(5) |
(8) |
(10) |
(4) |
|
|
| COX-1 |
10.45 ± 1.59 |
2.82 ± 0.90 |
3.40 ± 0.71 |
1.57 ± 0.34 |
7.04 ± 2.42 |
<0.0001 |
ns |
| COX-2 |
0.50 ± 0.21 |
0.27 ± 0.13 |
0.81 ± 0.21 |
1.02 ± 0.39 |
1.58 ± 0.68 |
ns |
ns |
| EGFR |
13.66 ± 1.26 |
4.90 ± 1.40 |
5.28 ± 0.89 |
3.37 ± 0.58 |
7.02 ± 0.83 |
<0.0001 |
ns |
|
Protein (n) |
(30) |
(5) |
(10) |
(10) |
(5) |
|
|
| COX-1 |
2.43 ± 0.19 |
1.97 ± 0.50 |
1.92 ± 0.38 |
2.98 ± 0.61 |
1.97 ± 0.50 |
ns |
ns |
| COX-2 total* |
1.24 ± 0.10 |
1.53 ± 0.36 |
3.57 ± 0.68 |
2.34 ± 0.26 |
3.42 ± 0.70 |
<0.0001 |
I,II 0.002 |
| |
|
|
|
|
|
|
I,IV 0.009 |
| COX-2 66 kDa |
1.00 ± 0.13 |
1.25 ± 0.33 |
1.63 ± 0.51 |
1.59 ± 0.32 |
1.62 ± 0.22 |
0.028 |
ns |
| COX-2 74 kDa |
0.20 ± 0.02 |
0.25 ± 0.09 |
1.70 ± 0.68 |
0.70 ± 0.22 |
1.49 ± 0.82 |
0.003 |
I,II 0.014 |
| |
|
|
|
|
|
|
II,III 0.036 |
| EGFR extra |
0.64 ± 0.06 |
0.91 ± 0.09 |
0.43 ± 0.12 |
0.40 ± 0.10 |
0.44 ± 0.15 |
ns |
I,II 0.009 |
| |
|
|
|
|
|
|
I,III 0.006 |
| |
|
|
|
|
|
|
I,IV 0.023 |
| EGFR intra | 0.54 ± 0.09 | 0.40 ± 0.19 | 0.14 ± 0.06 | 0.54 ± 0.16 | 0.42 ± 0.15 | ns | ns |
Mean ± SEM, RNA: units/units ((GAPDH + 18SRNA)/2), protein arbitrary units. *COX-2 total=66 kDa + ~74 kDa protein band in WB. EGFR intra./EGFR extra. = ab directed towards intracellular/extracellular EGFR protein.