Table 1.

Cell culture medium and supplements

Reagent	Suppliers	Concentration
EGF	Peprotech, Rocky Hill, NJ, USA	20 ng/ml
FGF-b	Peprotech	20 ng/ml
LIF	eBioscience, San Diego, CA, USA	20 ng/ml
B27	Invitrogen, Grand Island, NY, USA	20 μl/ml
BSA	Thermo Scientific HyClone, Logan, UT, USA	4 mg/ml
RPMI1640	Thermo Scientific HyClone	1×
DMEM/F12	Thermo Scientific HyClone	1×