Figure 3.

cAMP analogue produces an increase in I_A G_max. A. 50 μM 8-Bromo-cAMP (with or without rapamycin) was applied to an ongoing rhythm for 1 hr, followed by a 1 hr block and TEVC for I_A. Rapamycin was applied 10 min prior to 8-bromo-cAMP administration. These data were compared to 2 hr control, 5 nM DA, or 5 nM DA + RAP. B. The data for each treatment group ± S.D were plotted. Asterisks indicate significant differences from control. Data was analyzed using ANOVA’s with Dunnett’s post hoc tests. 8-Bromo cAMP persistently increased I_A G_max, while Rapamycin blocks or attenuates those effects.