Figure 4.
PKA antagonist Rp-cAMP blocks DA induced increase in IA Gmax. A. The specific PKA antagonist Rp-cAMP was applied in conjunction with TTX for 1 hr (with or without DA) followed by a 3 hr washout, and 1 hr block. Rp-cAMP and TTX were applied 10 min before administration of DA. B. 1 mM Rp-cAMP blocked the DA induced increase in IA Gmax, and was significantly different than TTX + DA alone, indicating that the persistent effect is dependent of PKA. Data were analyzed with ANOVAs, Tukey’s post hoc that makes all pairwise comparisons and plotted as mean ± S.D.