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. 2013 Nov 14;13:101. doi: 10.1186/1472-6750-13-101

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Copyright © 2013 Zhao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SDS-PAGE analysis of recombinant BGL-CBD in E. coli JM109(DE3). Lane M: protein marker, lane 1: cell-free extract of JM109(DE3) harboring pET-20b, lane 2: cell-free extract of JM109(DE3) harboring pET-BGL-Linker-CBD, lane 3: purified BGL-CBD (5 μg).