Table 1.

Purification of BGL-CBD from E. coli harboring pET-BGL-Linker-CBD

Steps	Total protein (mg)	Total activity (U)	Specific activity (U/mg)	Yield (%)
Cell extract	158	3357	21.2	100
Heat treatment	89	2954	33.2	88%
Ni-NTA affinity	32	2578	80.5	77%