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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Anal Chem. 2013 Aug 22;85(17):10.1021/ac400774g. doi: 10.1021/ac400774g

Figure 1. Experimental scheme of the high-throughput analyses of isolated plasma membranes of rat liver and hepatocellular carcinoma Morris hepatoma 7777.

Figure 1

Isolated plasma membranes from normal rat liver and hepatocellular carcinoma Morris hepatoma 7777 were pretreated with either 1) Salt (sodium carbonate) washing only or 2) sodium carbonate washing followed by trypsin predigestion to remove the soluble and peripheral membrane proteins. Subsequently, the resulting plasma membrane pellets were solubilized with detergents. Proteins were reduced, alkylated, and then directly incorporated into a polyacrylamide gel. The enhanced gel-assisted digestion was then performed. Peptides extracted from the gel were applied to LC-MS/MS analysis for protein identification and quantification. 3) Deglycosylation was performed after the formation of tube-gel to increase the protein sequence coverage for glycoprotein identification.