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. Author manuscript; available in PMC: 2014 Sep 3.
Published in final edited form as: Anal Chem. 2013 Aug 22;85(17):10.1021/ac400774g. doi: 10.1021/ac400774g

Figure 3. Histogram illustrations of the distributions of proteins with predicted TM helices identified from normal rat liver and hepatocellular carcinoma Morris hepatoma plasma membrane using different treatments.

Figure 3

Both data acquired by a QSTAR XL mass spectrometer (left) and an Orbitrap Velos mass spectometer (right) are shown for comparison. Identified proteins predicted with more than three TM helices are also shown in a more detailed view (below). Isolated plasma membranes were 1) LIPM Control: normal rat liver plasma membrane directly analyzed using the nonelectrophoretic gel-assisted digestion approach, 2) LISW or MHSW: LIPM or MHPM treated with carbonate washing, 3) LIPD or MHPD: LIPM or MHPM treated with carbonate washing followed by predigestion with trypsin. After the enhanced gel-assisted digestion, tryptic peptides were analyzed by LC-MS/MS for protein identification and quantification.