Table 1.
Control Values for Cholinergic and Serotonergic Biomarkers and for Behavioral Measures
| Cortex | ||||||
|---|---|---|---|---|---|---|
| PN10 | PN60 | |||||
| Female | Male | Combined | Female | Male | Combined | |
| AChE | 295.0±24.0 | 292.2±9.2 | 293.2±9.6 | 840.9±26.1 | 795.1±23.8 | 819.5±18.2 |
| ChAT | 0.23±0.02 | 0.22±0.01 | 0.22±0.01 | 1.40±0.05 | 1.26±0.06 | 1.33±0.04 |
| ChT | 43.4±1.6 | 44.2±1.5 | 43.8±1.1 | 103.5±4.6 | 111.2±3.7 | 107.3±3.0 |
| 5HT1A | 32.8±2.8 | 27.2±0.6 | 29.7±1.6 | 45.3±0.8 | 39.4±1.1 | 42.3±1.0 |
| 5HT2 | 29.3±1.5 | 23.2±0.9 | 25.9±1.3 | 59.6±1.7 | 56.4±2.0 | 58.0±1.3 |
| 5HTT | 139.3±3.6 | 141.1±7.6 | 140.3±4.3 | 183.2±5.3 | 172.0±7.2 | 177.6±4.5 |
| Brainstem | ||||||
| PN10 | PN60 | |||||
| Female | Male | Combined | Female | Male | Combined | |
| AChE | 432.7±16.7 | 385.9±4.9 | 401.5±8.5 | 100.5±3.5 | 97.5±2.2 | 98.9±2.0 |
| ChAT | 0.45±0.01 | 0.47±0.03 | 0.46±0.02 | 0.63±0.02 | 0.57±0.02 | 0.60±0.02 |
| ChT | 34.5±1.5 | 41.9±1.4 | 38.7±1.4 | 24.3±0.6 | 25.6±0.5 | 25.0±0.4 |
| 5HT1A | 12.9±1.2 | 14.7±0.7 | 14.1±0.7 | 23.2±0.7 | 22.1±0.7 | 22.7±0.5 |
| 5HT2 | 27.7±3.0 | 26.7±2.1 | 27.0±1.7 | 7.5±0.2 | 4.9±0.4 | 6.2±0.4 |
| 5HTT | 211.4±5.4 | 219.7±13.1 | 216.9±8.7 | 109.6±4.3 | 108.7±4.6 | 109.2±3.1 |
| Behavior | ||||
| Female | Male | Combined | ||
| EPM | %Time OA | 4.1±1.2 | 1.7±0.5 | 2.8±0.7 |
| %Entries OA | 10.5±2.9 | 6.8±2.1 | 8.6±1.8 | |
| Time Cen | 82.8±10.9 | 82.5±9.7 | 82.6±7.2 | |
| Entries CA | 9.6±1.2 | 8.9±0.9 | 9.2±0.7 | |
| OF | Ambulation OF | 1716.9±149.7 | 1830.5±151.8 | 1763.2±106.7 |
| Time Cen OF | 32.4±4.5 | 31.3±6.3 | 31.9±3.6 | |
| FST | Immob Time | 7.8±4.6 | 22.9±8.5 | 15.7±5.2 |
| PA | (L3 − L0)/L0 | 2.3±0.7 | 1.3±0.3 | 1.9±0.5 |
Note. Neurochemical biomarkers: AChE (nmoles/min/mg protein); ChAT (fmol/mg protein/min); ChT, 5HT1A, 5HT2, and 5HTT (fmol/mg protein). Behavioral measures: Time Cen (s); Ambulation (cm); Immob Time (s). PA, passive avoidance test; %Time OA, percentage of time spent in the open arms; %Entries OA, percentage of entries in the open arms; EntriesCA, number of entries in the closed arms; Time Cen, time spent in the center; Ambulation, traveled distance; Immob Time, immobility time; (L3 − L0)/L0, memory/learning index.