Fig. 2.

Thermal scanning of MDP (1 mg/mL) under three different solution conditions using ThT. The relative fluorescence units (RFU; Inset) were normalized and fit to Eq. 1 to obtain the mid-point of the thermal transitions. Inset shows the plot of the fluorescence intensity change as a function of temperature, and the error bars in black are the SD of the triplicate runs. The SD in T _M.agg measurement under these conditions in this case was ≤0.1°C