Figure 1. Gic1 scaffolds septin filaments resulting in long and flexible filament cables.
(A and B) Yeast septin octamers containing Cdc3-EGFP polymerized by dialysis alone (A) or together with Gic1 (B) and imaged using fluorescence microscopy. Scale bar, 0.5 µm. (C and D) Representative EM image of negatively stained septin filaments (C) and septin-Gic1 complexes (D) without EGFP. Scale bar, 100 nm. (E–G) Representative class averages with focus on the overall structure of the septin-Gic1 complex (E), the Gic1 cross-bridges (F) and the septin filaments (G); arrows indicate single septin proteins. Scale bars, 10 nm.