Figure 11. Septin polymerization depends on the ionic strength.
(A and B) Representative EM images and class averages of septin complexes at high-salt (500 mM NaCl) labeled with antibody against Cdc3 (C) and low-salt (100 mM NaCl) (D) conditions. Arrows indicate single septin proteins. Scale bars, B = 100 nm, D = 10 nm. (E) Model of the septin complex based on the known sequential order of septin filaments. The G- and the N/C-interfaces are indicated by straight and circular interfaces between circles, respectively.