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(A) Confirmation of the integration of the marker cassette in to the CgHOG1 locus. The genomic locus and primer binding sites is indicated. (B) C. glabrata Hog1 is required for osmotic stress tolerance. BG14, BG14hog1Δ, and BG14 carrying pHog1-GFP were diluted and spotted on YPD containing the indicated amount of NaCl. Growth was recorded after about 36 h.
