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. 2013 Dec;142(6):625–640. doi: 10.1085/jgp.201311045

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© 2013 Morgan et al.

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Figure 5. — Voltage and pH dependence of gating of hH_V1 mutants with the selectivity filter shifter from 112 to 116. Families of currents in a COS-7 cell expressing D112A/V116D channels at pH_i 6.5 and pH_o 7.5 (A), 6.5 (B), or 5.5 (C) are shown in 10-mV increments as labeled, from V_hold −60 mV (A) or −30 mV (B and C). Cells were returned to V_hold after pulses. (D) Current–voltage relationships in this cell. Currents were fitted to a rising exponential function and extrapolated to infinite time. Note inward currents at pH_o 5.5. (E) Conductance–voltage relationships for the same currents. Limiting slope conductance for the most negative voltages provides a gating charge estimate of 5.3–6.0 e₀. (F) Regulation of voltage gating by ΔpH. V_threshold is plotted against V_rev measured in the same cell and solution. Lines show linear regression fits defined by V_threshold = 0.64 V_rev + 0 mV (D112V/V116D; red circles) or V_threshold = 0.63 V_rev − 26 mV (D112A/V116D; blue diamonds).