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. 2013 Dec;142(6):625–640. doi: 10.1085/jgp.201311045

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© 2013 Morgan et al.

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Figure 6. — The D112V/V116E mutant is a functional proton-selective channel. (A) Families of currents generated by D112V/V116E in a HEK-293 cell in 10-mV increments at pH_o 5.5 and pH_i 5.5 (A), and pH_o 5.0 and pH_i 5.5 (B). As indicated, V_hold was −60 mV, and the membrane was returned to V_hold after pulses. (C) V_rev measured at pH_o 5.0, 5.5, 6.5, 7.0, and 7.5, and at pH_i 5.5 or 6.5, indicates that D112V/V116E is proton selective. Inset shows tail currents at 10 (uppermost), 5, and 0 mV at pH_o 5.5 and pH_i 5.5. Proton selectivity is imparted by either Asp or Glu at positions 112 or 116, indicating that side-chain length is not critical for this function.