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. 2013 Nov 25;203(4):575–583. doi: 10.1083/jcb.201306012

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© 2013 Zhang et al.

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Figure 2. — Atro is a repressor of Hh signaling pathway in D. melanogaster. (A) A cartoon of the wing discs from third-instar larva. The dashed line indicates the A/P compartment boundary. dpp expression region is shown in red. (B) Schematic shows that Hh gradient induces different target gene expression through transcriptional factor Ci. (C–E) A wild-type wing disc (C) or wing discs expressing Myc-tagged Atro (Myc-Atro; D) or Atro RNAi (E) with MS1096-Dicer2 Gal4 were immunostained to show the expression of dpp-lacZ. dpp-lacZ down-regulated in D (arrow) and up-regulated in E (arrows). (F and F′) A wing disc–expressing Myc-Atro with AG4-GFP was immunostained to show the expression of dpp-lacZ (red) and GFP (green). Atro-expressing clones (dashed lines) were marked by GFP-positive cells. (G and G′) A wing disc carrying Atro³⁵ mutant clones (marked by GFP-positive cells in dashed lines) was immunostained to show the expression of dpp-lacZ (red) and GFP (green). (H) A MS1096-Dicer2 Gal4 wing disc was immunostained to show wild-type Ci staining. (I–J″) Wing discs expressing Ci RNAi alone or Ci RNAi and Myc-Atro together with MS1096-Dicer2 Gal4 were immunostained to show the expression of dpp-lacZ (red), Ci (green), and Myc-tagged Atro (blue). All of the phenotypes shown are stable and representative under each of the indicated genetic conditions.