Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Nov 25;203(4):575–583. doi: 10.1083/jcb.201306012

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Atro recruits Rpd3 to repress Hh signaling. (A) S2 cells expressing the indicated proteins were harvested for the two-step immunoprecipitation and analyzed by Western blotting. (B) Rpd3 cooperates with Atro to repress Hh signaling in reporter assay in S2 cells (mean ± SD; n = 3). The whole DNA amount transfected in each group was normalized equal with blank vectors. (C–F) A wild-type wing disc (C) or wing discs expressing Rpd3-V5 (D), Rpd3 RNAi (E), or Rpd3 RNAi plus Myc-Atro (F) with MS1096-Dicer2 Gal4 were immunostained to show the expression of dpp-lacZ. The level of dpp-lacZ was reduced in Rpd3 overexpressing disc (D, arrows) and increased in Rpd3 knockdown disc (E, arrows). Atro overexpression failed to block the up-regulated expression of dpp-lacZ by Rpd3 knockdown (F, arrows). (G–G‴) A wing disc expressing Myc-Atro plus Rpd3 RNAi with AG4-GFP was immunostained to show the expression of dpp-lacZ– (red), GFP- (green), and Myc-tagged Atro (blue). (H–I‴) Wing discs carrying Atro³⁵ clones or Atro³⁵ plus Rpd3 overexpression were immunostained to show the expression of dpp-lacZ (red) and GFP (green). From G to I‴, clones were marked by GFP-positive cells (dashed lines).