Table 1.
qRT-PCR analysis of differentially expressed genes of An. gambiae in ONNV infection
| Gene ID (GenBank Accession No.) | Primer sequence (5′ to 3′) | Fold change (P-value, SD) | Amplicon size (bp) | Amplification efficiencya (Rb) |
|---|---|---|---|---|
| RpS4 (AJ283756) | Forward: GCTGCCGCTGGTGATCTT | |||
| Reverse: TCGTCACCTCGCTGTTGGT | 1.2 (0.259, 0.42) | 65 | 0.90 (0.99) | |
| AdeL (AL692204) | Forward: CGTGCAGAATGGCCGAA | |||
| Reverse: CGTGCTCAGCGGCGA | 5.4 (0.031, 3.0) | 59 | 0.90 (0.99) | |
| RpL35 (BX466552) | Forward: GCAACCAGCACGAGAAGCA | |||
| Reverse: GAACAGGACGTTCTTGCGGT | 3.5 (0.002, 0.96) | 68 | 1.1 (0.99) | |
| Hsp 70 (AL930714) | Forward: GCGATCCAGGCCGACAT | |||
| Reverse: TCTTTGGCTTGCCCTCGAT | 29.2 (0.02, 16.4) | 64 | 0.97 (0.99) | |
| Dan4 (AL694117) | Forward: GGTAGGAAAAATGCGGGACAA | |||
| Reverse: TTATCGTGCAGCCGCAATC | 6.0 (0.004, 3.24) | 66 | 1.1 (0.99) | |
| EF-1α (N/Dc) | Forward: GGACAGATCAGCAACGGATACA | |||
| Reverse: TCGGAGAACTTGCACGCA | 4.8 (0.01, 0.10) | 73 | 1.1 (0.99) | |
| Agglutinin (N/D) | Forward: CGCTGACCGCTACGGTGT | |||
| Reverse: AATCGGCTGTTCTCGTTGGA | 6.4 (0.01, 1.14) | 67 | 0.91 (0.99) | |
| MRpL7 (N/D) | Forward: GGTGGTGAAGACGACGTTCA | |||
| Reverse: CCTTGATCAGGGCCACCTT | −2.0 (0.04, 0.09) | 71 | 1.1 (0.99) |
a
Amplification efficiencies were calculated from the slope of standard curves as E = 10[−1/slope] − 1. 100% PCR efficiency corresponds to an amplification efficiency of 1 (Applied Biosystems Application Note);
b
Regression coefficient of linear standard curve.
c
Accession number has not yet been designated.