Fig. 4. Assessment of transfection efficiency with SDF-1α in human SkM. The highest transfection and expression of SDF-1α in presence of 125μM ZnCl2 were achieved with FuGeneTM6. ELISA for SDF-1α level was monitored for 12 days (pg of hSDF-1α/mg of total protein)in the supernatant and cell lysate (A-B) from SkM transfected with FuGeneTM6/phSDF-1α in the presence and absence of ZnCl2. Immunostaining of SkM for SDF-1α overexpression in vitro after transfection with FuGeneTM6/phSDF-1α at day 4 (C).
