Figure 1.

Schematic overview of the cleavable linker approach.

A) DUBs are labeled with an ABP containing the cleavable linker. The labeled proteins are retrieved on a streptavidin matrix which is then washed before release by selective cleavage of the linker.

B) Schematic overview of probe delivery approach. Cells are incubated on ice with Perfringolysin-O in the presence of the probe. The cells are transferred to 37°C to allow pore formation and are incubated for 30 min to allow labeling to occur. The cytosol is then separated from the cellular compartments by centrifugation.