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. 2013 Oct 2;1(5):e00096. doi: 10.1002/phy2.96

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© 2013 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Mating and diet scheme required to produce generation of F2 iron-sufficient (F2 IS) from formerly iron-deficient (FID) rats. Pregnant dams (P0) were given iron-deficient (ID) diet beginning at gestational day (G) 2. Nursing dams and F1 pups continued on an ID diet until postnatal day (P) 7, at which time both were switched to an IS diet. F1 rats were weaned at P21. At P90, when no longer iron-deficient (formerly iron-deficient) F1 were allowed to mate to produce F2 pups. Hippocampal RNA was collected and evaluated from F1 and F2 rats at the P15 and P65 time points shown in bold.